AmyA anticorps

N° du produit ABIN99685

N° du produit (Fournisseur): 100-101-234

Cet anticorps Chèvre Polyclonal détecte spécifiquement dans ELISA et WB. Il présente une réactivité envers Rhizopus.

Aperçu rapide pour AmyA anticorps (ABIN99685)

Antigène: AmyA

Reactivité: Rhizopus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 100-101-234

Fournisseur: Rockland

Fonction: Glucoamylase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucoamylase [Rhizopus].

Attributs du produit: Synonyms: goat anti-Glucoamylase Antibody, 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Glucoamylase [Rhizopus]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Application Note: Anti-Glucoamylase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucoamylase [Rhizopus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: AmyA

Sujet: Background: Rhizopus glucoamylase exists in multiple forms, Gluc 1, Gluc 2, and Gluc 3, all of which hydrolyze gelatinized starch at similar rates, but only the largest one (Gluc 1) is able to adsorb raw starch. Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

UniProt: P07683